家畜人工授精に於ける抗菌性諸物質の應用 : 第I報 保存精液に於ける精子生存時間に及ぼす抗菌性諸物質の影響
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The effect of several antibiotics on the spermlongivity of the rabbit semen which was preserved in room temperature was observed. Acetosulfamin, penicillin G, Streptomycin and Streptothricin group substances (No.36 substance, Seki-2, Seki-3, and Seki-7 substances) were used as the antibiotics.The semen-diluter was compenoated with the phosphate buffer solution to three groups of pH 7.6, 7.0 and 6.5 respectively. The antibiotics were contained in each these groups of preserved semen as follows. The density of Acetsulfamin was mede up 0.5mg×10-1, 0.5mg×10-2, 0.5mg ×10-3, 0.5mg×10-4, and 0.5mg×10-5 per ml of diluted semen, and those of the others were made up 1mg 10-1, 1mg×10-2, 1mg×10-3, 1mg ×10-1, and 1mg×10-5 per ml. As the control spermlongivity of the preserved semen was observed without carried on in sterilized condition, so there were no effect of bacteria.The average spermlongivities cf diluted semen without respect to the density of antibiotics were shown in the table.1) In the case of pH 7.6, the sperm lived longer in Penicillin G, streptomycin, Seki-3 substance and No. 36 substance in control. In Seki-2 substance, the lengivity was equal to control, 86 and in Acetsulfamin or SEKI-7 substance, it was shorter than in control. Particularly, SEKI-7 substance had a toxity for spermatozoa.2) In the case of pH 7.0, the longest spermlongivity was observed in SEKI-2 substance. In Acetsulfarmin, No. 36 substance and Streptomycin it was longer than in control, , In Penicillin G and Seki-7 substance, it was SRELATIVELY-3 shorter hanin control, and the shortest was in SEKI-3 tsubs- tance.3) In the case of pH 6.5, all the spermlongivity of the preserved semen containing these antibiotics were shorter than the control, and it was remarkably shorter in SEKI-3 and SEKI-7 substance.4) Feing compared one another those three kinds of pH the spermlongivity was considerably longer in pH 7.0 than in the cases of pH 7.6 and 6.5. The shortest was in pH 7.6.5) The antibiotics used, except SEKI-7 substance had no toxity for spermatozoa in the case of pH 7.0 and 7.6, and SEKI-3 substance showed, however, a little toxic effect on spermatozoa in the case of pH 7.0.Those results above mentioned suggest that six kinds of those antibiotics, namely Acetsulfamin, Penicillin G, Streptomycin, No. 36 substance, Seki -2 substance and Siki-3 substance may be able to applied to artificial insemination of domestic animals, if the condition of the medium is considered.Particularly, the application of Streptomycin, No. 36 substance and Seki-2 substance, of which actions in keeping the motility of spermatozoa suitably appear in the neutral or the basic, may give a satisfactory result
- 社団法人 日本畜産学会の論文