馬の人工授精に關する研究 : (第III) 報精蟲の-新保存法, 所謂馬研法に就て
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As formerly reported, expected results were not obtained with various dilution media of spermatozoa. Hereafter, we found a new method “Baken Method” after various basic investigations. Baken is the abbreviation of the former name of this institute. Baken Method is divided into No.1 and No.2.<BR>a. Baken Method No.1<BR>1) Procedure: 8-16 cc of horse sperm added with 1-2 cc of Baken solution No.1 is centrifuged for 10-15 minutes with 1, 000-1, 500 revolutions per minute. After removal of sperm serum, 1-2 cc of the same solution is added to the layer of spermatozoa and then mixed slightly. The tube which contains thus treated sperm is wrapped with gauze and rubber sack and preserved in a thermostat at 4°C. The preparation of the Baken Solution No.1 is as follows: 100 cc distilled water+0.08g KCl + 0.05g Na2HPO<SUB>4</SUB>·12H<SUB>2</SUB>O, 95 cc above solution+5g glucose+3cc fresh egg yolk.<BR>2) Test for viability of spermatozoa preserved by this method is much better than the others.<BR>3) Fertilizing ability of spermatozoa preserved for about six hours by this method were tested at this institute in 1945, and in two districts in Hokkaido in 1945 and 1946 where about 2-3 hours were required to transport the sperm to the destination by train. The results were not generally inferior to those of the notural mating.<BR>b. Baken Method No.2<BR>This method was designed with the chject of omitting the centrifugation procedure in Baken Method No.1. The raw sperm are diluted 4-8 times with Baken Solution No.2 and preserved in a thermostat at 4°C. The composition of this solution is as follows: 100 cc distilled water+0.025g KCl+0.05g Na<SUB>2</SUB>HP<SUB>4</SUB>·12H<SUB>2</SUB>O, 95 cc of above solution+5g glucose+2.5cc fresh egg yolk. Results of the viability test with this solution are more or less inferior than those of Method No.1 in a long preservation, but in about six hours preservation the second method showed almost the same results as the former.
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