酵素―リガンド複合体のコンホメーション変化の速度論的解析
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概要
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Conformational change occurring in the enzyme and/or enzyme-ligand complexes has been suggested, and evidences for it have been obtained by X-ray crystallography for some enzymes.<BR>In the kinetic studies of enzyme-ligand interactions by the temperature-jump and stopped-flow methods, unimolecular isomerization processes of enzymes and/or enzyme-ligand complexes are frequently observed, which are attributable to a conformational change.<BR>Unimolecular isomerization processes may be classified as follows, A) Conformational change of the enzyme only E_??_E* B) Conformational change of the enzyme-ligand complexes E·S_??_E·S* E·S_??_E*·S E·S_??_E*·S* where the mark * shows the "deformated state" of the enzyme or the ligand (E= enzyme, and S=ligand).<BR>Examples for each case are reviewed for several enzymes including serine proteinases, lysozyme, RNAse, and aminoacylt-RNA synthetase.
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