大腸菌ファージfdのプロモーター領域の構造
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概要
- 論文の詳細を見る
In order to define the primary structure in promoter, two high level promoters contained in bacteriophage fd DNA have been sequenced. Comparison of two sequences however indicated that the longest sequence common to both was only TATAAT in the non-transcribed part of the region where RNA-polymerase forms a stable initiation complex. One of the promoters sequenced contained an R. Hha I cleavage site five base pairs upstream from the TATAAT sequence, and cleavage destroyed promoter function. This promoter was cleaved into two pieces at this site and DNA fragments derived from other regions were joined to the resulting fragments. Ligation of any fragment examined to the TATAAT-containing fragments restored promoter function. On the basis of these observations, the structure essential for the function of fd promoter is discussed.
- 日本生物物理学会の論文