The Studies on the Transfection of EGFP-Fused Porcine Circovirus Type 2 Genome

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概要

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• The study described the construction and transfection of EGFP-fused Porcine Circovirus Type 2 (PCV2) genome and the recovery of the virus. Post-transfection, PCV2 (ORF1)-EGFP/pSK, PCV2 (ORF3)-EGFP/pSK, PCV2 (ORF4)-EGFP/pSK, and PCV2(ORF5)-EGFP/pSK showed no fluorescent signals in transfected cells, while green fluorescent signals were observed in the nucleus of PK-15 cells after PCV2 (ORF2)-EGFP/pSK transfection. The presence of ORF2-EGFP fusion protein was demonstrated by dual signals of green fluorescence and anti-PCV2 antibodies conjugated with rhodamine in IFA. Furthermore, the released EGFP-fused PCV2 genome was demonstrated by real-time PCR.

著者

• CHEN Hsu-Chung

  School of Veterinary Medicine, National Taiwan University

• YANG Shu-Hsiang

  Institute of Biotechnology, National Ilan University

• KUO Tsun-Yung

  Institute of Biotechnology, National Ilan University

• LAI Shiow-Suey

  School of Veterinary Medicine, National Taiwan University

関連論文

• The Studies on the Transfection of EGFP-Fused Porcine Circovirus Type 2 Genome

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