Differentiation of Foot-and-Mouth Disease-Infected Pigs from Vaccinated Pigs Using Antibody-Detecting Sandwich ELISA

概要

論文の詳細を見る
The presence of serum antibodies for non-structural proteins of the foot-and-mouth disease virus (FMDV) can differentiate FMDV-infected animals from vaccinated animals. In this study, a sandwich ELISA was developed for the rapid detection of the foot-and-mouth disease (FMD) antibodies; it was based on an Escherichia coli-expressed, highly conserved region of the 3ABC non-structural protein of the FMDV O/TW/99 strain and a monoclonal antibody derived from the expressed protein. Diagnostic sensitivity of the assay was 98.4% and the diagnostic specificity was 100% for naïve and vaccinated pigs and the detection ability of the assay was comparable to kits of the PrioCHECK and UBI. The agreement between results obtained from our ELISA and those from the PrioCHECK, UBI, and CHEKIT was 97.5, 93.4, and 66.6%, respectively. The kappa statistics were 0.95, 0.87, and 0.37, respectively. Moreover, antibodies for non-structural proteins of the serotypes A, C, Asia 1, SAT 1, SAT 2, and SAT 3 were also detected in bovine sera. Furthermore, the absence of cross-reactions generated by different antibody titers against the swine vesicular disease virus and vesicular stomatitis virus (VSV) was also highlighted in this assays specificity.