Evaluation of Different Cryoprotectants (CPAs) in Boar Semen Cryopreservation
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概要
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This study was performed to evaluate the use of dimethylacetamide (DMA) and dimethyl sulfoxide (DMSO) in boar sperm cryopreservation. Semen from eight boars was cryopreserved following treatment with 3, 5, and 7% DMA and DMSO, and 3% glycerol (control). After thawing, sperm conventional parameters and membrane integrities were evaluated. There were no significant differences among different DMA concentrations in all evaluations. Membrane intactness were higher in 5% and 7% DMSO than 3% DMSO (P<0.05). Sperm motility of 5% DMSO was lower than that of 3% glycerol (P<0.005), and membrane intactness were lower in 5% DMA and DMSO than 3% glycerol (P<0.05). DMA and DMSO didnt improve sperm quality and glycerol remains the most useful for boar sperm cryopreservation.
著者
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LEE Young-Jun
Department of Electronics & Electrical Engineering
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LEE Young-Jun
Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University
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KIM Yong-Jun
Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University
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KIM Suhee
Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University
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JI Dong-Beom
Department of Veterinary Obstetrics and Theriogenology, College of Veterinary Medicine, Chonbuk National University
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- Evaluation of Different Cryoprotectants (CPAs) in Boar Semen Cryopreservation