Development and Application of a Chemically Defined Medium for the In Vitro Production of Porcine Embryos

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概要

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• Recent advances in systems for in vitro production (IVP) of porcine embryos, including in vitro oocyte maturation, fertilization and embryo culture, have enabled us to generate viable embryos that can develop to full term after transfer into recipients. This technology is being applied now to developments in gamete/embryo biology and agriculture, as well as in producing cloned and genetically modified pigs. Chemically defined media for IVP of embryos are useful for a precise analysis of the physical action of substances on gametogenesis and early embryogenesis, because they eliminate undefined factors present in biological materials, such as serum or serum albumin. Use of a chemically defined medium also improves the reliability of media formulations, yields a higher reproducibility of results and ensures biosafety of culture media by eliminating protein preparations, which may be contaminated with pathogens. Therefore, it has certain advantages for research and for commercial purposes. We have recently developed a defined IVP system for porcine embryos using a single basic medium based on the composition of porcine oviductal fluid. This paper discusses the developmental ability and normality of porcine IVP embryos, and limitations and advancements in this system.

• 日本繁殖生物学会の論文

著者

• Yoshioka Koji

  Research Team For Production Diseases National Institute Of Animal Health

関連論文

• Successful Piglet Production in a Chemically Defined System for In-vitro Production of Porcine Embryos : Dibutyryl Cyclic AMP and Epidermal Growth Factor-family Peptides Support In-vitro Maturation of Oocytes in the Absence of Gonadotropins
• Development and Application of a Chemically Defined Medium for the In Vitro Production of Porcine Embryos
• Development and Application of a Chemically Defined Medium for the In Vitro Production of Porcine Embryos

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