A Strong Protective Action of Guttiferone-A, a Naturally Occurring Prenylated Benzophenone, Against Iron-Induced Neuronal Cell Damage
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概要
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Guttiferone-A (GA) is a natural occurring polyisoprenylated benzophenone with several reported pharmacological actions. We have assessed the protective action of GA on iron-induced neuronal cell damage by employing the PC12 cell line and primary culture of rat cortical neurons (PCRCN). A strong protection by GA, assessed by the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carbox-anilide (XTT) assay, was revealed, with IC50 values <1 μM. GA also inhibited Fe3+–ascorbate reduction, iron-induced oxidative degradation of 2-deoxiribose, and iron-induced lipid peroxidation in rat brain homogenate, as well as stimulated oxygen consumption by Fe2+ autoxidation. Absorption spectra and cyclic voltammograms of GA–Fe2+/Fe3+ complexes suggest the formation of a transient charge transfer complex between Fe2+ and GA, accelerating Fe2+ oxidation. The more stable Fe3+ complex with GA would be unable to participate in Fenton-Haber Weiss-type reactions and the propagation phase of lipid peroxidation. The results show a potential of GA against neuronal diseases associated with iron-induced oxidative stress.
著者
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Figueredo Yanier
Laboratorio de Farmacología Molecular, Centro de Investigación y Desarrollo de Medicamentos, Cuba
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García-Pupo Laura
Laboratorio de Farmacología Molecular, Centro de Investigación y Desarrollo de Medicamentos, Cuba
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Rubio Osmany
Departamento de Farmacia, Instituto de Farmacia y Alimentos, Universidad de La Habana, Cuba
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Hernández René
Laboratorio de Farmacología Molecular, Centro de Investigación y Desarrollo de Medicamentos, Cuba
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Naal Zeki
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidad
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Curti Carlos
Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidad
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Andreu Gilberto
Centro de Estudio para las Investigaciones y Evaluaciones Biológicas, Instituto de Farmacia y Alimen
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Garcia-pupo Laura
Laboratorio De Farmacologia Molecular Centro De Investigation Y Desarrollo De Medicamentos
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Curti Carlos
Departamento De Fisica E Quimica Faculdade De Ciencias Farmaceuticas De Ribeirao Preto Universidade
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Hernandez Rene
Laboratorio De Farmacologia Molecular Centro De Investigation Y Desarrollo De Medicamentos
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Rubio Osmany
Departamento De Farmacia And Instituto De Farmacia Y Alimentos Universidad De La Habana
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Andreu Gilberto
Centro De Estudio Para Las Investigaciones Y Evaluaciones Biologicas Instituto De Farmacia Y Aliment
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Figueredo Yanier
Laboratorio De Farmacologia Molecular Centro De Investigation Y Desarrollo De Medicamentos
関連論文
- A Strong Protective Action of Guttiferone-A, a Naturally Occurring Prenylated Benzophenone, Against Iron-Induced Neuronal Cell Damage
- A Strong Protective Action of Guttiferone-A, a Naturally Occurring Prenylated Benzophenone, Against Iron-Induced Neuronal Cell Damage