Inhibitory Effects of Dexamethasone on Hepatocyte Growth Factor–Induced DNA Synthesis and Proliferation in Primary Cultures of Adult Rat Hepatocytes

スポンサーリンク

概要

• 論文の詳細を見る

• We investigated the effects of dexamethasone on hepatocyte growth factor (HGF)-induced DNA synthesis and proliferation in serum-free primary cultures of adult rat hepatocytes. Isolated hepatocytes were cultured at a density of 3.3 × 104 cells/cm2 in Williams’ medium E containing 5% newborn bovine serum and various concentrations of dexamethasone for 1, 2, and 3 h. After a 3-h attachment period, the medium was then changed, and cells were cultured in serum-free dexamethasone (10−10 M)-containing Williams’ medium E with or without glucocorticoid receptor antagonists. After addition of dexamethasone to the culture medium, the growth-stimulating effects of HGF (5 ng/mL) on the primary cultured hepatocytes were time- and dose-dependently inhibited. The mineralcorticoid aldosterone (10−7 M) did not produce the same growth-inhibitory effects as dexamethasone (10−8 M). The inhibitory effects of dexamethasone were reversed by treatment with the glucocorticoid-receptor antagonist mifepristone (RU486, 10−6 M) or a monoclonal antibody against glucocorticoid receptor (100 ng/mL). In addition, the growth-inhibitory dose of dexamethasone did not affect HGF-induced receptor tyrosine kinase and extracellular signal-regulated kinase 2 phosphorylation. These results indicate that dexamethasone dose-dependently delays and inhibits HGF-induced DNA synthesis and proliferation through its own intracellular receptor in primary cultures of adult rat hepatocytes.

• 社団法人 日本薬理学会の論文

著者

• Ogihara Masahiko

  Department Of Clinical Pharmacology Faculty Of Pharmaceutical Sciences Josai University

• KIMURA Mitsutoshi

  Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Josai University

• Kimura Mitsutoshi

  Department Of Clinical Pharmacology Faculty Of Pharmaceutical Sciences Josai University

• Moteki Hajime

  Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Josai University, Japan

• Moteki Hajime

  Department Of Clinical Pharmacology Faculty Of Pharmaceutical Sciences Josai University

関連論文

• Expression of α_2- and β_2-Adrenergic Responses by Hepatocyte Growth Factor and Platelet-Derived Growth Factor in Primary Cultures of Adult Rat Hepatocytes
• Prolonged liver dysfunction caused by hemosiderosis in a renal transplant recipient
• IP Receptor Agonist-Induced DNA Synthesis and Proliferation in Primary Cultures of Adult Rat Hepatocytes : the Involvement of Endogenous Transforming Growth Factor-α
• Activation of Mitogen-Activated Protein Kinase by Hepatocyte Growth Factor Is Stimulated by Both α_1- and β_2-Adrenergic Agonists in Primary Cultures of Adult Rat Hepatocytes
• Inhibitory Effects of Dexamethasone on Hepatocyte Growth Factor–Induced DNA Synthesis and Proliferation in Primary Cultures of Adult Rat Hepatocytes
• Inhibitory Effects of Dexamethasone on Epidermal Growth Factor-Induced DNA Synthesis and Proliferation in Primary Cultures of Adult Rat Hepatocytes
• Activation of Extracellular-Signal Regulated Kinase by Platelet-Derived Growth Factor Is Potentiated by Phenylephrine in Primary Cultures of Adult Rat Hepatocytes
• Activation of extracellular-signal regulated kinase by epidermal growth factor is potentiated by cAMP-elevating agents in primary cultures of adult rat hepatocytes
• Activation of Extracellular-Signal Regulated Kinase by Epidermal Growth Factor Is Potentiated by cAMP-Elevating Agents in Primary Cultures of Adult Rat Hepatocytes
• Inhibitory Effects of Dexamethasone on Hepatocyte Growth Factor-Induced DNA Synthesis and Proliferation in Primary Cultures of Adult Rat Hepatocytes
• Hemodynamic influence of tilting disc valve type on pump performance with the NIPRO-ventricular assist device
• Metabolic alterations in normal and streptozotocin-diabetic rats in vivo: Influence of prolonged starvation.

もっと見る 閉じる

スポンサーリンク