Down-Regulation of Vascular Endothelial Growth Factor and Up-Regulation of Pigment Epithelium Derived Factor Make Low Molecular Weight Heparin-Endostatin and Polyethylene Glycol-Endostatin Potential Candidates for Anti-angiogenesis Drug

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概要

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• The aim was to study the effects and action mechanism of endostatin (ES), low molecular weight heparin-endostatin (LMWH-ES) and polyethylene glycol-endostatin (PEG-ES) on endothelial cell proliferation, choroidal neovascularization and zebrafish angiogenesis. Three-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide was used to study the effects of ES and its derivatives on endothelial cell proliferation in vitro. Choroidal neovascularization model was used to evaluate the effects of ES and its derivatives on choroidal neovascularization in vivo. Western blotting was employed to study the effects of ES and its derivatives on the expression of vascular endothelial growth factor (VEGF) and pigment epithelium derived factor (PEDF) in chorioid tissues. Zebrafish model was also used to study the anti-angiogenesis activities of ES and its derivatives. The results showed that ES and its derivatives could significantly inhibit endothelial cell proliferation in vitro (p<0.05), suppress choroidal neovascularization by down-regulating expression of VEGF and up-regulating expression of PEDF in chorioid tissues, and restrain angiogenesis in zebrafish. ES showed better activity in inhibiting endothelial cell proliferation in vitro (p<0.05), but LMWH-ES and PEG-ES showed higher activity in inhibiting choroidal neovascularization in vivo (p<0.05) and angiogenesis in zebrafish (p<0.05). These results indicate that LMWH-endostatin and PEG-endostatin are potential candidates for anti-angiogenesis drug.

著者

• Wang Fengshan

  Institute Of Biochemical And Biotechnological Drug School Of Pharmaceutical Science Shandong Univers

• TAN Haining

  Institute of Biochemical and Biotechnological Drug, School of Pharmaceutical Science, Shandong Unive

• MU Guoying

  Jinan Central Hospital and Clinical Medical College of Shandong University

• Tan Haining

  Institute Of Biochemical And Biotechnological Drug School Of Pharmaceutical Science Shandong Univers

• Zhu Wei

  Coll. Of Animal Sci. Guizhou Univ.

• Zhu Wei

  Jinan Central Hospital Affiliated to Shandong University and Clinical Medical College of Shandong Un

• Liu Jinfeng

  Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Shandong Uni

• Zhu Wei

  Jinan Central Hospital Affiliated to Shandong University and Clinical Medical College of Shandong University

• Mu Guoying

  Jinan Central Hospital Affiliated to Shandong University and Clinical Medical College of Shandong University

• Wang Fengshan

  Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Shandong University

関連論文

• Characterization and Secondary Structure Analysis of Endostatin Covalently Modified by Polyethylene Glycol and Low Molecular Weight Heparin
• Down-Regulation of Vascular Endothelial Growth Factor and Up-Regulation of Pigment Epithelium Derived Factor Make Low Molecular Weight Heparin-Endostatin and Polyethylene Glycol-Endostatin Potential Candidates for Anti-angiogenesis Drug

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