サンプル混合液を用いた定量ウェスタンブロット解析用検量線の作成

スポンサーリンク

概要

• 論文の詳細を見る

• For accurate protein quantification when using quantitative western blot analysis with chemiluminescence reagents, standard curves are needed because of the narrow quantifiable ranges. However, they are often difficult to obtain because authentic proteins are not always available. Here we present our original and convenient method using a sample mixture as a scale to create standard curves. This method allowed us to determine the quantifiable range of target and loading control proteins, making quantitative comparisons among independent blots more reproducible. Our results indicate that using a sample mixture to create standard curves is a practical method that guarantees the accuracy and reproducibility of quantitative western blot analysis.

• 社団法人　日本実験動物学会の論文

著者

• 野口 洋子

  理化学研究所 バイオリソースセンター

• SUZUKI Osamu

  Laboratory of Animal Models for Human Diseases, National Institute of Biomedical Innovation

• KOURA Minako

  Laboratory of Animal Models for Human Diseases, National Institute of Biomedical Innovation

• NOGUCHI Yoko

  Laboratory of Animal Models for Human Diseases, National Institute of Biomedical Innovation

• UCHIO-YAMADA Kozue

  Laboratory of Animal Models for Human Diseases, National Institute of Biomedical Innovation

• MATSUDA Junichiro

  Laboratory of Animal Models for Human Diseases, National Institute of Biomedical Innovation

• Matsuda Junichiro

  Laboratory Of Animal Models For Human Diseases National Institute Of Biomedical Innovation

関連論文

• Intracerebral cell transplantation therapy for murine GM1 gangliosidosis
• サンプル混合液を用いた定量ウェスタンブロット解析用検量線の作成
• Research Resources Bank for Laboratory Animals at the National Institute of Biomedical Innovation : overview
• Adult onset cardiac dilatation in a transgenic mouse line with Galβ1,3GalNAc α2,3-sialyltransferase II (ST3Gal-II) transgenes: a new model for dilated cardiomyopathy
• サンプル混合液を用いた定量ウェスタンブロット解析用検量線の作成
• Increased globotriaosylceramide levels in a transgenic mouse expressing human α1,4-galactosyltransferase and a mouse model for treating Fabry disease
• Zygosity Determination in Hairless Mice by PCR Based on Hrhr Gene Analysis

スポンサーリンク