Large-Scale Production of Porcine Mature Interleukin-18 (IL-18) in Silkworms Using a Hybrid Baculovirus Expression System.

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概要

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• In this report, a hybrid baculovirus expression system, which means a hybrid virus of the Autographa californica nuclear polyhedrosis virus and the Bombyx mori nuclear polyhedrosis virus, was used for the large-scale production of porcine mature interleukin-18 (IL-18) in silkworms. Two recombinant hybrid baculoviruses containing cDNA of the porcine precursor IL-18 and the porcine caspase-1 were constructed and were used to infect silkworm larvae. After the co-infection of the two viruses, porcine mature IL-18 was efficiently produced in the haemolymph. The concentration of IL-18 in the haemolymph was 80-100 μg/ml, as determined by porcine IL-18 specific ELISA. This yield was twenty-times more than that of the insect cell expression system described previously. The porcine mature IL-18 produced by the silkworms strongly induced interferon-γ (IFN-γ) production from porcine PBMC. An insect factory system for the large-scale production of useful cytokines for livestock animals will be available in the near future.

• 公益社団法人 日本獣医学会の論文

著者

• MORI Yasuyuki

  Department of Immunology, National Institute of Animal Health

• MUNETA Yoshihiro

  Department of Immunology, National Institute of Animal Health

• INUMARU Shigeki

  Department of Immunology, National Institute of Animal Health

• ZHAO Hong

  Shandong Agricultural University

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• AN ASSESSMENT OF THE PRODUCTION OF MOUSE GERM-LINE CHIMERAS DERIVED FROM EMBRYONIC STEM CELLS INTRODUCED INTO 8-CELL-STAGE HOST EMBRYOS BY CO-CULTURE AND MICROINJECTION METHODS
• Large-Scale Production of Porcine Mature Interleukin-18 (IL-18) in Silkworms Using a Hybrid Baculovirus Expression System.

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