Bovine Apolipoprotein E in Plasma: Increase of ApoE Concentration Induced by Fasting and Distribution in Lipoprotein Fractions.
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概要
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Apolipoprotein E (apoE) is a protein constituent of lipoproteins, and acts as a receptor-binding ligand. Although the existence of bovine apoE in lipoprotein fractions has already been reported, quantitative studies on the changes of apoE in plasma and lipoprotein fractions are lacking. In the present study, an increase of a 38 kDa protein in the very low-density lipoprotein (VLDL) fraction obtained from fasted calves was detected. This 38 kDa protein was identified as bovine apoE by determination of the N-terminal amino acid sequence. Bovine apoE was purified and an enzyme-linked immunosorbent assay (ELISA) was developed. Using this system, the effect of fasting on the concentration of apoE in plasma and the distribution of apoE in lipoprotein fractions were investigated. After 3 days of fasting, the concentration of plasma apoE increased significantly (p<0.05) by 280 %, and was returned to the basal level by 3 days of refeeding. The lipoprotein fractions obtained from before and after fasting was separated by ultracentrifugation. ApoE was significantly increased in VLDL, low-density lipoprotein (LDL) and non-lipoprotein fractions by fasting (p<0.05). On the other hand, in high-density lipoprotein (HDL) fractions obtained from both before and after fasting, the level of apoE was very low compared to the other fractions. These results suggested that bovine apoE contents in triglyceride-rich lipoproteins are modulated by nutritional treatment and closely associated with triglyceride-rich lipoprotein metabolism.
- 公益社団法人 日本獣医学会の論文
著者
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ITOH Fumiaki
National Institute of Livestock and Grassland Science
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Sato Kan
Laboratory Of Animal Nutritiion Graduate School Of Agricultural Science Tohoku University
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Katoh Norio
Hokkaido Research Station National Institute Of Animal Health
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SATO Kan
Laboratory of Animal Nutrition, Tohoku University
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OOHASHI Tsutai
National Institute of Animal Health
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TAKAHASHI Yuji
National Institute of Animal Health
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MIYAMOTO Toru
National Institute of Animal Health
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