Prevalence of Tick-Borne Rickettsia and Ehrlichia in Ixodes persulcatus and Ixodes ovatus in Tokachi District, Eastern Hokkaido, Japan
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概要
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DNA from 111 ticks collected by flagging in Tokachi district, Eastern Hokkaido, Japan were examined for infection with Rickettsia and Ehrlichia, by PCR and sequencing methodology. For Rickettsia, analysis of the partial sequence of the citrate synthase gene was successfully performed on 11 DNA samples from I. persulcatus, and 7 of them showed 99.8% identical with Rickettsia helvetica while the other 4 showed 99.8% identical with `Candidatus Rickettsia tarasevichiae. For Ehrlichia, a partial sequence of the 16S rRNA gene detected from I. persulcatus was 100% identical with that from Ehrlichia muris, and another DNA sample from I. ovatus showed 99.8% identical with Ehrlichia species detected from I. ovatus. The results suggest that the pathogens detected here might be distributed in this area.
著者
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INOKUMA Hisashi
Obihiro University of Agriculture and Veterinary Medicine
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OHASHI Misato
Obihiro University of Agriculture and Veterinary Medicine
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TANABE Shigeyuki
Obihiro University of Agriculture and Veterinary Medicine
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MIYAHARA Kazuro
Obihiro University of Agriculture and Veterinary Medicine
関連論文
- Prevalence of Tick-Borne Rickettsia and Ehrlichia in Ixodes persulcatus and Ixodes ovatus in Tokachi District, Eastern Hokkaido, Japan
- Serological Survey of Neospora caninum Infection among Dogs in Japan through Species-Specific ELISA