Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR

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概要

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• A rapid decrease in the concentration of microcystin due to the decline of Microcystis spp. cells has been observed during fall in Japan. Past researches have shown the involvement of microcystin-degrading bacteria in this phenomenon, but the process by which it occurs has not yet been elucidated. In this research, microcystin-degrading bacteria were quantified using real-time TaqMan polymerase chain reaction. The new TaqMan probe was based on the sequence of the mlrA gene that is conserved in microcystin-degrading bacteria; new primers were similarly developed. These new primers and probe enabled the precise examination of microcystin-degrading bacteria in a biofilm. Moreover, the bacteria present in a biofilm from a practical biological treatment facility could be detected and quantified. The results showed that microcystin-degrading bacteria existed in the biofilm throughout the year, and the number of bacterial cells increased in fall.

著者

• Shimizu Kazuya

  Toyo Univ. Gunma Jpn

• UTSUMI Motoo

  University of Tsukuba, Japan

• JIMBO Yusuke

  University of Tsukuba, Japan

• OKANO Kunihiro

  Akita Prefectural University, Japan

• MASEDA Hideaki

  University of Tokushima, Japan

• FUJIMOTO Naoshi

  Tokyo University of Agriculture, Japan

• SUGIURA Norio

  University of Tsukuba, Japan

• SHIMIZU Kazuya

  Toyo University, Japan

関連論文

• Quantification of Microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR
• Application of Oxygen Productive Electrode (OPE) in water treatment
• Characterization of 2-MIB-degrading bacteria isolated from biofilm in drinking water facility
• Biodegradation of microcystins by the bacterial communities symbiotic with microflagellate Monas guttula
• Isolation of 2-MIB-degrading bacteria from biofilm in drinking water facility
• Application of a New Electrolysis Cell to Toxic Cyanobacteria Inhibition in Water Environment

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