DNA Damage and Decrease of Cellular Oxidase Activity in Piglet Sertoli Cells Exposed to Arsanilic Acid

概要

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The study was designated to explore the toxic effects of arsanilic acid on piglet Sertoli cells. Sertoli cells were isolated from piglet testes using a two-step enzyme digestion and followed by differential plating. Piglet Sertoli cells were cultured and classified into five groups, i.e., group A, the control without arsanilic acid treatment; group B with 5 μM arsanilic acid; group C with 50 μM arsanilic acid; group D with 0.5 mM arsanilic acid; and group E with 5 mM arsanilic acid. We found that Sertoli cells growth was inhibited by arsanilic acid at 0.5 mM compared with the control group A. The oxidase activity of Sertoli cells was decreased by arsanilic acid at 0.5 mM as evidenced by the observations that arsanilic acid increased MDA content but decreased SOD and GSH-Px activities of Sertoli cells. Moreover, DNA damage of Sertoli cells was observed by arsanilic acid at 50 μM. Considered together, our study suggests that exposure of arsanilic acid to Sertoli cells leads to an induction of oxidative stress and an inhibition of cell growth at a high concentration, while arsanilic acid results in DNA damage of Sertoli cells at a low concentration.