Timing of Completion of the First Meiotic Division in Bovine Oocytes after Maintenance of Meiotic Arrest with Cycloheximide and Their Subsequent Development.

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概要

• 論文の詳細を見る

• This study examined the timing of completion of meiosis I of bovine oocytes in which meiotic resumption had been inhibited by cycloheximide (CHX), and also determined the optimum interval of maturation in culture for subsequent fertilization and development. Most oocytes treated with CHX reached metaphase II at 16 hr in the maturation culture, while control oocytes did at 20 hr. CHX-treated oocytes cultured for 16 hr were normally fertilized but failed to develop into blastocysts. Maturation in culture for 20 hr resulted in comparable development for control oocytes. The results indicate that nuclear maturation of CHX-treated oocytes was completed 4 hr faster than for control oocytes, however the same interval of maturation as that of control oocytes is necessary for subsequent development to blastocysts.

• 公益社団法人 日本獣医学会の論文

著者

• Nagai Masaki

  Embryo Transplantation Laboratory Snow Brand Milk Products Co. Ltd.

• KISHI Masao

  Embryo Transplantation Laboratory

• SAEKI Kazuhiro

  Research Institute for Biology-Oriented Science and Technology, Kinki University

• IRITANI Akira

  Research Institute for Biology-Oriented Science and Technology, Kinki University

• KISHI Masao

  Embryo Transplantation Laboratory , Snow Brand Milk Products Co., Ltd.

• NAGAO Yoshikazu

  Embryo Transplantation Laboratory , Snow Brand Milk Products Co., Ltd.

関連論文

• 形質転換ウシ受胎産物の作出:PCR法による形質転換胚の選別の可能性
• In vitro development of bovine nuclear transfer embryos reconstructed with mammary gland epithelial cells at different passages
• Timing of Completion of the First Meiotic Division in Bovine Oocytes after Maintenance of Meiotic Arrest with Cycloheximide and Their Subsequent Development.

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