A Micromethod for Protein Assay Using a Light Microscope.

スポンサーリンク

概要

• 論文の詳細を見る

• A micromethod which utilizes a protein-dye binding reaction on an acetate membrane and a light microscope with an automatic exposure instrument has been developed for measuring small amounts of protein from limited biological materials. After discoidal cellulose acetate membranes (2.0 mm diameter), which absorbed 0.5 μl of protein solution containing 0.2-2.0 μg of protein, were stained with Coomassie Brilliant Blue G-250, they were examined by a light microscope with an automatic exposure instrument. A linear relation was confirmed between the intensity of dye binding to protein and exposure time under specific optical conditions.

• 公益社団法人 日本獣医学会の論文

著者

• Matsuzawa Tokihiro

  Laboratory Of Biology Obihiro University Of Agriculture And Veterinary Madicine

• TAKEKOSHI Miyoko

  Laboratory of Biology, Obihiro University of Agriculture and Veterinary Medicine

• AIKAWA Jiroh

  Laboratory of Biology, Obihiro University of Agriculture and Veterinary Medicine

関連論文

• Electrophoretic and Histochemical Studies on Hepatic 3α-Hydroxysteroid Dehydrogenase in the Rat
• Substrate Specificity and Histochemical Distribution of Aminopeptidase in Rat Testis and Epididymis
• Staining Specificity of Histochemical Reaction for 3β-Hydroxysteroid Dehydrogenase Activity in Mouse Testis
• A Micromethod for Protein Assay Using a Light Microscope.

スポンサーリンク