種々の温度で培養した冷水病菌のVBNC状態への移行
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概要
- 論文の詳細を見る
Bacterial cold-water disease (BCWD) caused by Flavobacterium psychrophilum leads to heavy mortality of ayu Plecoglossus altivelis in Japan. Previously, a 28℃ warmed water treatment was shown to be an effective treatment for BCWD, since F. psychrophilum can not grow and maintain colonies at this temperature. However, it was unclear whether the bacteria might resuscitate after the treatment was over. Therefore, we investigated colony formation, membrane potential and pathogenicity of F. psychrophilum at various temperatures (15℃, 23℃, 28℃ and 33℃) in sterilized underground water. Within 2 days at 28℃, F. psychrophilum completely lost their colony-forming abilities but still maintained their membrane potentials. It seemed that these cells entered into viable but non-culturable (VBNC) state. However, experimental ayu infection revealed that VBNC F. psychrophilum cells were unable to cause BCWD, suggesting that the cells were progressing towards death at 28℃.
- 日本魚病学会の論文
著者
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菅原 和宏
Shiga Prefectural Fisheries Experimental Station
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永田 恵里奈
Department Of Fisheries Kinki University
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Eguchi Mitsuru
Department Of Fisheries Faculty Of Agriculture Kinki University
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Fujiwara-nagata Erina
Department Of Fisheries Faculty Of Agriculture Kinki University
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福田 文美
Graduate School Of Agriculture Kinki University
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Sugahara Kazuhiro
Shiga Prefectural Fisheries Experimental Station
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Fukuda Ayami
Graduate School of Agriculture, Kinki University
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Fujiwara-Nagata Erina
Department of Fisheries, Kinki University
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- 種々の温度で培養した冷水病菌のVBNC状態への移行
- Direct Detection of a Fish Pathogen, Vibrio anguillarum Serotype J-0-1, in Freshwater by Fluorescent Antibody Technique
- 種々の温度で培養した冷水病菌のVBNC状態への移行