Construction of a Binary Vector for Transformation of Arabidopsis thaliana with a New Selection Marker
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概要
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A new binary vector, pZT4B, containing the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as a selection marker, was constructed. The green fluorescent protein (GFP) gene was inserted into pZT4B, and the resulting plasmid was used in the transformation of Arabidopsis. All of six independent transformants obtained after selection with 0.3 mg/l tunicamycin contained the transgene and showed GFP fluorescence.
- 社団法人 日本農芸化学会の論文
著者
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Iwata Yuji
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Koizumi Nozomu
Graduate School Of Life And Environmental Sciences Osaka Prefecture University
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- Construction of a Binary Vector for Transformation of Arabidopsis thaliana with a New Selection Marker
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