β-Glucosidase Catalyzed Syntheses of Pyridoxine Glycosides

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概要

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• Enzymatic syntheses of pyridoxine glycosides were carried out in di-isopropyl ether organic medium using β-glucosidase isolated from sweet almond. Optimum conditions determined for the reaction with D-glucose were 40% (w/w D-glucose) β-glucosidase at 0.18 mM (1.8 ml) of pH 5 acetate buffer over a 72 h incubation period. Of 11 carbohydrates employed, β-glucosidase gave 7-O-(α-D-glucopyranosyl)pyridoxine 5a, 7-O-(β-D-glucopyranosyl)pyridoxine 5b, 6-O-(α-D-glucopyranosyl)pyridoxine 5c, 7-O-(α-D-galactopyranosyl)pyridoxine 6a, 7-O-(β-D-galactopyranosyl)pyridoxine 6b, 6-O-(α-D-galactopyranosyl)pyridoxine 6c, 7-O-(α-D-mannopyranosyl)pyridoxine 7a, 7-O-(β-D-mannopyranosyl)pyridoxine 7b, and 6-O-(α-D-mannopyranosyl)pyridoxine 7c in yields ranging from 23 to 40%.

• 社団法人 日本農芸化学会の論文

著者

• Charles Rajachristu

  Fermentation Technol. And Bioengineering Dep. Central Food Technological Res. Inst. Council Of Scien

• Divakar Soundar

  Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute

関連論文

• β-Glucosidase Catalyzed Syntheses of Pyridoxine Glycosides
• β-Glucosidase Catalyzed Syntheses of Pyridoxine Glycosides

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