Molecular Characterization of a TIA-1-Like RNA-Binding Protein in Cells Derived from the Fall Armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

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概要

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• A complementary DNA encoding a TIA-1-type RNA-binding protein (SfTRN-1) was isolated from cultured cells of the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), to characterize its function. The deduced 388-amino acid sequence of SfTRN-1, which possessed three RNA recognition motifs (RRMs) followed by a C-terminal auxiliary domain, showed significant homology with mammalian TIA-1/TIAR and silkworm BmTRN-1, factors important in the metabolism of transcripts. It was found that inhibition of SfTRN-1 gene expression by a transfected oligonucleotide encoding the antisense sequence led to a marked increase in the production of a reporter protein and the amount of reporter transcript in the cultured cells. In addition, overexpression of the recombinant full-length SfTRN-1 open reading frame in the cultured cells led to a decrease in reporter protein production, but the truncated RRM1-3 domain lacking the C-terminal auxiliary domain lost its activity. Analysis using a GFP-fused recombinant protein revealed that, unlike mammalian TIA-1/TIAR, SfTRN-1, most likely shuttling between the nucleus and cytoplasm, had the characteristic of being largely distributed in the cytoplasm, where it perhaps acts to reduce the amount of transcripts, and that RRM1 and RRM3 were related to its nuclear accumulation, but RRM2 to its nuclear export. Furthermore, the posterior half of the auxiliary domain was also found to be related to its nuclear export. This study indicates that respective RRM subdomains of SfTRN-1 play distinct roles important to its subcellular distribution, and it identified unique systems for the distribution and functional regulation of the TIA-1 family in insect cells, ones which are clearly different from those in mammalian cells.

• 社団法人 日本農芸化学会の論文

著者

• MUTO Sayaka

  Graduate School of Science and Technology, Kyoto Institute of Technology

• MATSUMOTO Emi

  Graduate School of Science and Technology, Kyoto Institute of Technology

• TANABE Toru

  Graduate School of Science and Technology, Kyoto Institute of Technology

• MORI Hajime

  Graduate School of Science and Technology, Kyoto Institute of Technology

• KOTANI Eiji

  Graduate School of Science and Technology, Kyoto Institute of Technology

• Muto Sayaka

  Graduate School Of Science And Technology Kyoto Institute Of Technology

関連論文

• Functional analysis of the gene of BmTRN-1, an RNA-binding protein homologous to mammalian TIA-1 from the silkworm, Bombyx mori ; a study of it's overexpression and sub-cellular distribution during the baculovirus infection process
• Embryonic Development in the Eggs of the Silkworm, Bombyx mori, Exposed to the Space Environment
• Molecular Characterization of a TIA-1-Like RNA-Binding Protein in Cells Derived from the Fall Armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)
• Purification and Properties of Double-stranded RNA-degrading Nuclease, dsRNase, from the Digestive Juice of the Silkworm, Bombyx mori
• Identification of Novel Double-stranded RNA Produced in Midgut Epithelial Tissue of the Silkworm, Bombyx mori, during Infection by a Cypovirus 1
• Induction of Genomic DNA Fragmentation by Introduction of the BmTRN-1 RNA-Binding Domain into Cultured Cells of the Silkworm, Bombyx mori
• Somatic Mutation in Larvae of the Silkworm, Bombyx mori, Induced by Heavy Ion Irradiation to Diapause Eggs
• Efficient Induction of Shoot Organogenesis From Leaves of Mulberry Seedlings Using 2,3,5-Triiodobenzoic Acid
• カイコ幼虫での多糖類投与による抗菌活性の誘導評価
• Molecular Characterization of a TIA-1-Like RNA-Binding Protein in Cells Derived from the Fall Armyworm Spodoptera frugiperda (Lepidoptera : Noctuidae)
• Characterization of a novel C-type lectin cDNA, CLEM 20 cDNA, specifically expressed in mouthparts of the flesh fly Sarcophaga peregrina

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