Superoxide Scavenging and Xanthine Oxidase Inhibiting Activities of Copper–β-Citryl-L-glutamate Complex

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概要

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• β-Citryl-L-glutamate (β-CG) is a unique compound initially isolated from developing brains, which also appears in high concentrations during the period characterized by growth and differentiation of neurons in developing animals, and then decreases with maturation. However, its functional roles remain unclear. The stability constant obtained in our previous pH titration studies showed that β-CG forms relatively strong complexes with copper. Reactive oxygen species (ROS) and nitric oxide (NO) have been suggested to act as mediators of the cell death that occurs in neurons during development of the nervous system. However, regulation of ROS and NO formation by Cu in the developing brain remains poorly understood. The activity of superoxide dismutase (SOD), a key superoxide scavenging enzyme, is low in the developing brain. Furthermore, xanthine oxidase (XO) has been implicated in diverse pathological situations due to its capability of generating both ROS and NO. Therefore, we examined the effects of β-CG and its Cu-complex on SOD and XO activities. We found that the [Cu(II)(β-CG)] complex had SOD activity and a strong competitive inhibition of XO, while reduced glutathione caused concentration-dependent decreases of the XO inhibitory activities in the [Cu(II)(β-CG)] complex.

著者

• Narahara Masanori

  Faculty Of Pharmaceutical Sciences Kobe Gakuin University

• Narahara Masanori

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University

• Hamada-Kanazawa Michiko

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University

• Kouda Makiko

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University

• Odani Akira

  Division of Pharmaceutical Sciences, Graduate School of Natural Science and Technology, Kanazawa Uni

• Miyake Masaharu

  Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kobe-Gakuin University

• Odani Akira

  Division Of Pharmaceutical Sciences Graduate School Of Natural Science And Technology Kanazawa Unive

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