Involvement of Glutamine-238 in the Substrate Specificity of Human Laeverin/Aminopeptidase Q
スポンサーリンク
概要
- 論文の詳細を見る
Human laeverin/aminopeptidase Q (APQ) is a novel member of the M1 family of zinc aminopeptidases and is specifically expressed on the cell surface of extravillous trophoblasts. In this study, we examined the significance of Gln-238 of laeverin/APQ, a putative S1 site residue, by site-directed mutagenesis for its enzymatic activity and substrate specificity. Replacement of Gln-238 with Ala caused a significant change in substrate specificity rather than a decrease in enzymatic activity. These results indicate that Gln-238 is important for the substrate specificity of laeverin/APQ. In addition, our data suggest that direct electrostatic interaction between substrate and S1 site of the enzyme is not involved in the mutant enzymes preference for basic amino acids.
著者
-
Goto Yoshikuni
Laboratory of Cellular Biochemistry, RIKEN
-
Yoshioka Rina
Laboratory of Cellular Biochemistry, RIKEN
-
Arisaka Naomi
Laboratory of Cellular Biochemistry, RIKEN
-
Hattori Akira
Department of System Chemotherapy and Molecular Sciences, Graduate School of Pharmaceutical Sciences
-
Tsujimoto Masafumi
Laboratory of Cellular Biochemistry, RIKEN
-
Tsujimoto Masafumi
Laboratory Of Cell Biochemistry Riken
-
Hattori Akira
Department Of Internal Medicine Sado Kouseiren Hospital
-
Hattori Akira
Department of System Chemotherapy and Molecular Sciences, Graduate School of Pharmaceutical Sciences, Kyoto University
関連論文
- Involvement of glutamine-238 in the substrate specificity of human laeverin/aminopeptidase Q
- Involvement of Glutamine-238 in the Substrate Specificity of Human Laeverin/Aminopeptidase Q
- Genomic Organization of the Human Adipocyte-Derived Leucine Aminopeptidase Gene and Its Relationship to the Placental Leucine Aminopeptidase/Oxytocinase Gene^1
- Characterization of Recombinant Human Adipocyte-Derived Leucine Aminopeptidase Expressed in Chinese Hamster Ovary Cells^1
- Molecular Cloning of Adipocyte-Derived Leucine Aminopeptidase Highly Related to Placental Leucine Aminopeptidase/Oxytocinase
- Ligand-binding Activity and Expression Profile of Annexins in Caenorhabditis elegans
- Structural Changes in the Glycosaminoglycan Chain of Rat Skin Decorin with Growth
- 耐熱合金のBr_2-O_2-Ar雰囲気における腐食
- Processing of Antigenic Peptides by Aminopeptidases(Aminopeptidases in Health and Disease)
- Comparison of the Enzymatic Properties of Mouse β-Galactoside α2, 6-Sialyltransferases, ST6Gal I and II
- Asymmetric Distribution of Phospholipids in Biomembranes(Lipid Dynamics and Pathobiology in Membrane Lipid Rafts)
- Foreword(Aminopeptidases in Health and Disease)
- Vascular thromboxane formation in hemostasis mechanism: correlation between bleeding time and vascular TXB_2 in a patient with congenital platelet cyclo-oxygenase deficiency
- Features of Japanese patients with myelodysplastic syndrome in an aging population of Sado Island
- Exon 10 Coding Sequence Is Important for Endoplasmic Reticulum Retention of Endoplasmic Reticulum Aminopeptidase 1
- Molecular Cloning of Adipocyte-Derived Leucine Aminopeptidase Highly Related to Placental Leucine Aminopeptidase/Oxytocinase.