DNA-based characterization of an invasive mealybug (Hemiptera: Pseudococcidae) species damaging cotton in Pakistan
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概要
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Mealybug (Hemiptera: Pseudococcidae) has emerged as a new cotton pest in Pakistan in recent years. We used DNA nucleotide sequences and PCR-RFLP, respectively, as tools for mealybug characterization and species composition. Partial nucleotide sequences of nuclear (elongation factor-1 α, ribosomal DNA subunits 18S and 28S) and mitochondrial (COI) genes were used for species characterization, and the combined ITS1-5.8S-ITS2 sequences of rDNA were used for PCR-RFLP analyses. Homology searches of the nuclear genes indicated that the mealybug species damaging cotton in Pakistan belonged to the genus Phenacoccus. Further, the barcoding sequence of COI showed a significant nucleotide similarity with Phenacoccus solenopsis. PCR-RFLP analysis with three different endonucleases did not show restriction site differences among mealybug individuals collected from various host plants and geographical locations. This is the first DNA-based characterization of cotton mealybug from Pakistan and the findings will help in decision making while considering a biological control program.
- Japanese Society of Applied Entomology and Zoologyの論文
著者
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Ashfaq Muhammad
National Institute for Biotechnology and Genetic Engineering (NIBGE), Pakistan
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Noor Ali
National Institute for Biotechnology and Genetic Engineering (NIBGE), Pakistan
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Mansoor Shahid
National Institute for Biotechnology and Genetic Engineering (NIBGE), Pakistan
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Ashfaq Muhammad
National Inst. For Biotechnology And Genetic Engineering (nibge) Pak
関連論文
- DNA-based characterization of an invasive mealybug (Hemiptera: Pseudococcidae) species damaging cotton in Pakistan
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