メンブランフィルタとガラスフィルタを組み合わせた有機物分解菌の分離·計数用の平板培地の開発
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概要
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A new plate culture system, designated as GM plate, for isolation and enumeration of bacteria decomposing organic substance in water has been developed. The GM plate was constructed as follows : a glass-fiber filter was placed in a petri dish and soaked with liquid medium containing 2.0g organic substance as carbon source (acetate, glucose, saccharose, raffinose, triacetin, dextrin, veratric acid, glycolate, starch, pectic acid, alginate or sodium carboxymethyl cellulose), 1.53g KNO3, 1.6g K2HPO4, 0.2g KH2PO4, 0.1g NaCl, 0.2g MgSO4·7H2O, 0.02g CaCl2·2H2O, 1.0mg FeSO4·7H2O, 1.0mg ZnSO4·7H2O and 1.0mg MnSO4·4-6H2O per litre, and a membrane filter was attached on the surface of glass-fiber filter. Sample solution was spread over the surface of the membrane filter of GM plate using spiral plating method. Seven days after incubation of the GM plate at 25°C, the colonies on the plate were counted by direct visual inspection. The method was simple in the operation, and released amounts of total organic carbon from the GM plate to the liquid phase were only 5% of that from agar plate.Although the pollution due to organic substances among water samples from three rivers investigated could not be distinguished by conventional agar plate method (total bacteria at 25 and 35°C and number of coliform groups, fungi and yeast) and physicochemical analysis (atmospheric and water temperature, pH, suspended solid, dissolved oxygen, biochemical oxygen demand, total organic carbon, total nitrogen and total phosphorus), significant differences in bacterial numbers and distributions were seen among three water samples by GM plate method. It seemed that quantity and quality of organic substances in the river influence the bacterial numbers and strains decomposing organic substances.These results indicated that the GM plate method is sensitive and useful for evaluating organic pollution in river water.
- 社団法人 日本水環境学会の論文
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関連論文
- メンブランフィルタとガラスフィルタを組み合わせた有機物分解菌の分離·計数用の平板培地の開発
- 河川における各種の有機物分解菌と汚濁との関連
- 河川における水質汚濁と細菌相との関連
- 河川水中の細菌数測定のための,スパイラル・プレーティング法の最適条件の統計学的検討