Musca domestica Larva Lectin Induces Apoptosis in BEL-7402 Cells through a Mitochondria-Mediated Reactive Oxygen Species Way
スポンサーリンク
概要
- 論文の詳細を見る
A lectin from Musca domestica larva was purified by affinity chromatography on a glactose-Sepharose 4B column. Musca domestica larva lectin (MLL) inhibited the growth of BEL-7402 cells in a time and concentration-dependent way. The results of Hoechst 33258 staining indicated that MLL induce BEL-7402 cells apoptosis based on the typical apoptotic morphological changes. Subsequently, we found that MLL treatment abrogated glutathione antioxidant system and induced mitochondria to generate reactive oxygen species (ROS) accumulation, resulting in reduction of mitochondrial transmembrane potential. The induction of cell apoptosis was caused by the upregulation of Bax, the downregulation of Bcl-2, the cytochrome c release and the activation of the caspases pathways.
著者
-
Zhao Qi
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and
-
Cao Xiaohong
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and
-
Zeng Bin
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and
-
Wang Chunling
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and
-
Yan Le
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and
-
Xu Chengjian
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and
-
Cao Xiaohong
Key Lab. Of Food Safety And Sanitation Ministry Of Education Coll. Of Food Engineering And Biotechno
-
Wang Chunling
Key Lab. Of Food Safety And Sanitation Ministry Of Education Coll. Of Food Engineering And Biotechno
-
Yan Le
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology
-
Zeng Bin
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology
-
Zhao Qi
Key Laboratory of Food Safety and Sanitation, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology
関連論文
- Musca domestica Larva Lectin Induces Apoptosis in BEL-7402 Cells through a Mitochondria-Mediated Reactive Oxygen Species Way
- A D-galactose-binding lectin with mitogenic activity from Musca domestica pupae