A Real-Time SUMO-Binding Assay for the Analysis of the SUMO-SIM Protein Interaction Network

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概要

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• SUMO-interacting motifs (SIMs) play a central role in the fate of SUMO-modified proteins. Here we report a real-time SUMO-binding assay. It can be applied to the identification of SIMs and to screening for the identification of novel SUMO-binding proteins. Using this assay, we investigated the SIMs in SETDB1 and MCAF1 to gain insight into the assembly of SETDB1-MCAF1-mediated gene silencing.

著者

• Tanaka Niina

  Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University

• Saitoh Hisato

  Department of Biological Sciences, Graduate School of Science and Technology, Kumamoto University

関連論文

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