Carbon and Nitrogen Isotope Analysis for Amino Acids from Biological Sample (質量分析連合討論会シンポジウム--生物活動と地球環境-2-)

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Evaluation was made for carbon and nitrogen stable isotope analyses of amino acids which were hydrolyzed from biological samples. Alteration of isotopic compositions during chemical preparations was studied in incorporation with organic solvent, acid hydrolysis, and ion-exchange chromatography. Based on such assessment, analyses of isotope compositions of single amino acids were made for collagen and plant protein. The carbon isotope composition of soybean protein showed consistent pattern with algal amino acids. Similarity was also found in the intermolecular relationship of δ13C and δ15N between collagen and soybean protein.
日本質量分析学会の論文