Ontogenic Change of Nitrogen Accumulation and Natural 15N Abundance in Pea and Faba Bean with Special Reference to Estimate of N2 Fixation and 15N Enrichment of Nodules (質量分析連合討論会シンポジウム--生物活動と地球環境)

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Nitrogen accumulation and changes in natural 15N abundance (δ15N) in five pea cultivars and two faba bean cultivars were investigated in an alluvial soil in the field during winter and spring. The amounts of nitrogen accumulated in tops were 16 to 20 g m-2 in pea plants, and 17 and 24 g m-2 in faba bean at final harvest. About 67~97% of the nitrogen in pea plants and 79 and 86% in faba bean were derived from atmospheric N2 based on the difference of δ15N values between N2-fixing legumes and the reference plant (barley was used).The nodules in most of the pea, faba bean, and vetch plants were enriched in 15N (up to +8‰). The 15N enrichment was more obvious in mature and senescent nodules, and the soluble N fraction of the nodule cytosol was most highly enriched in 15N. The δ15N values of ammonia extracted from the nodule bacteroids were higher than those of the organic N in this organelle and of ammonia in the nodule cytosol. The mechanisms of 15N enrichment in nodules were discussed.
日本質量分析学会の論文