緑色蛍光を発する先体部分と赤色蛍光を発する尾部を持つトランスジェニック精子の作製と先体反応の輸卵管内におけるin vivoイメージング
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概要
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In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and oviductal walls when female reproductive tracts were dissected out and placed under excitation light. This characteristic could facilitate examination of sperm migration inside the female reproductive tract as well as facilitating in situ live imaging of the acrosome reaction, the details of which have remained elusive.
著者
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蓮輪 英毅
Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
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室 悠子
Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
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伊川 正人
Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
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KATO Noriko
Laboratory of Molecular Genetics, Department of Medical Genetics, Osaka University Medical School
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TSUJIMOTO Yoshihide
Laboratory of Molecular Genetics, Department of Medical Genetics, Osaka University Medical School
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OKABE Masaru
Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
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Ikawa Masahito
Genome Information Res. Center Res. Inst. For Microbial Diseases Osaka Univ.
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HASUWA Hidetoshi
Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
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MURO Yuko
Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
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