妊娠マカクザル血漿を用いたネストPCRによる胎仔性別判定
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概要
- 論文の詳細を見る
Non-invasive fetal sex determination is required for biomedical studies, in which some sexual difference would be expected in fetal events, in order to make a choice of male or female fetus. To detect male fetal DNA of the sex-determining region Y gene (SRY) in maternal macaque plasma, nested real-time PCR using the SYBR Green system was developed. In all cases of pregnant macaques with male fetuses, a nested PCR product of SRY was amplified from the mother’s plasma, while no amplicon was detected in any case of pregnancy with a female fetus. Interestingly, fetal SRY DNA appeared to be cleared rapidly from the maternal blood after parturition. The current method is sensitive and can be performed with a regular PCR machine.
- 社団法人 日本実験動物学会の論文
著者
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Nakamura Shin
Department Of Food And Nutrition Beppu University
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Morimoto Mayumi
Department of Pediatrics, Shiga University of Medical Science
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Mitsunaga Fusako
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University
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Nakamura Shin
Department Of Cardiology Iwate Prefectural Iwai Hospital
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UEIWA Miyuki
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University
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KAMANAKA Yoshirou
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University
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Morimoto Mayumi
Department Of Pediatrics Shiga University Of Medical Science
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Mitsunaga Fusako
Department Of Cellular & Molecular Biology Primate Research Institute Kyoto University
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光永 総子
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University
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上岩 美幸
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University
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釜中 慶朗
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University
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MORIMOTO Mayumi
Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University
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