Three Cry Toxins in Two Types from Bacillus thuringiensis Strain M019 Preferentially Kill Human Hepatocyte Cancer and Uterus Cervix Cancer Cells
スポンサーリンク
概要
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Bacillus thuringiensis strain M019, non-pathogenic to lepidopteran and dipteran insects, produces a parasporal inclusion that consists of three 84-kDa Cry proteins (CPs). CP78A and CP78B, which exhibit 83.5% amino acid identity, were new variants of the previously reported HeLa cell-killing protein (parasporin-1). CP84 was a novel CP showing low-level homology, of 21.9% (56.4% similarity), with the insecticidal Cry2 toxin. In vitro solubilization with dithiothreitol at pH 10.2 and limited hydrolysis with trypsin resulted in the removal of N-terminal portions of the CPs and their activation. The 70-kDa proteins (15- and 55-kDa fragments) from CP78A and CP78B and the 73-kDa protein (14- and 59-kDa fragments) from CP84 exhibited varying degrees of cytocidal activity preferentially toward human hepatocyte cancer HepG2 cells and uterus cervix cancer HeLa cells causing cell swelling or the formation of vacuoles in the cytoplasm. These toxins appeared to attack an identical target on human cells.
著者
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Nagamatsu Yasunori
Graduate School of Biosphere Science, Hiroshima University
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Okamura Shoutarou
Graduate School of Biosphere Science, Hiroshima University
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Saitou Hiroyuki
Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
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AKAO Tetsuyuki
Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
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MIZUKI Eiichi
Biotechnology and Food Research Institute, Fukuoka Industrial Technology Center
関連論文
- Three Cry Toxins in Two Types from Bacillus thuringiensis Strain M019 Preferentially Kill Human Hepatocyte Cancer and Uterus Cervix Cancer Cells
- Failure to phenotypically discriminate between non-insecticidal Bacillus thuringiensis strains with anticancer parasporins (PS2, PS3, and PS4) and Bacillus thuringiensis strains that produce insecticidal Cry proteins
- A unique morphological feature commonly associated with Bacillus thuringiensis serovar yunnanensis isolates: An electron microscopic observation
- Three Cry Toxins in Two Types from Bacillus thuringiensis Strain M019 Preferentially Kill Human Hepatocyte Cancer and Uterus Cervix Cancer Cells