A Standardized Aqueous Extract of Anoectochilus formosanus Ameliorated Thioacetamide-Induced Liver Fibrosis in Mice: The Role of Kupffer Cells

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概要

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• Anoectochilus formosanus is used in traditional folk medicine as an hepatoprotective agent. The purpose of this study was to investigate the effects of a standardized aqueous extract of A. formosanus (SAEAF) on thioacetamide (TAA)-induced liver fibrosis. An in vitro study showed that the inhibitive effect of kinsenoside, a major component of SAEAF, on tumor necrosis factor α (TNF-α) secretion from Kupffer cells might be derived at least partly from downregulation of LPS-receptor Toll-like receptor 4 (TLR4) signaling. Hepatic fibrosis was produced by TAA (200 mg/kg, i.p.) 3 times per week for 12 weeks. Mice in the three TAA groups were treated daily with distilled water and SAEAF (1.0, 0.2 g/kg) via gastrogavage throughout the experimental period. The mice that received the SAEAF treatment had significantly reduced plasma alanine aminotransferase activity, relative liver weights, and hepatic hydroxyproline contents. A histological examination also confirmed that SAEAF reduced the degree of fibrosis caused by TAA treatment. RT-PCR analysis showed that SAEAF treatment reduced mRNA expression of collagen (α1)(I), lipopolysaccharide-binding protein, CD14, TLR4, and TNF receptor 1. An immunohistochemical examination also indicated that SAEAF reduced the number of CD68-positive cells (macrophages). In conclusion, oral administration of SAEAF significantly reduced TAA-induced hepatic fibrosis in mice, probably through inhibition of hepatic Kupffer cell activation.

• 社団法人 日本農芸化学会の論文

著者

• WU Jin-Bin

  Graduate Institute of Pharmaceutical Chemistry, China Medical University

• CHUANG Hin-Ru

  Graduate Institute of Basic Medical Science, China Medical University

• YANG Li-Chan

  School of Medicine, China Medical University

• LIN Wen-Chuan

  School of Medicine, Graduate Institute of Basic Medical Science and Tsuzuki Institute for Traditiona

関連論文

• A Standardized Aqueous Extract of Anoectochilus formosanus Ameliorated Thioacetamide-Induced Liver Fibrosis in Mice: The Role of Kupffer Cells
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• A Standardized Aqueous Extract of Anoectochilus formosanus Ameliorated Thioacetamide-Induced Liver Fibrosis in Mice : The Role of Kupffer Cells

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