Quantitative Analyses of CD133 Expression Facilitate Researches on Tumor Stem Cells

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概要

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• CD133 is regarded as a marker of tumor initiating cells in many tumors, including colorectal cancer. OBrien and Ricci et al. have proved that in primary colorectal tumors there are colorectal tumor stem cells (initiating cells) which are marked by CD133 antigen. Using a genetic knockin lacZ reporter mouse model, Shmelkov et al. challenged this increasingly influential viewpoint and drew two important conclusions that challenge former opinions. First, CD133 is widely distributed throughout the full range of tumor epithelial cells in the colon as opposed to being limited to a few cells. Second, CD133 negative cells of colon tumors are also tumorigenic, and are more inclined to metastasize. Based on these two opinions, we hypothesize that the expression of CD133 is different among tumor cells, and that quantitative but not qualitative analyses of CD133 abundance are necessary to determine the relationship between CD133 expression and tumor stem cell characteristics. To verify this hypothesis, colorectal cancer cell line SW620 was cultured and sorted into CD133Hi, CD133Mid and CD133Low subgroups using magnetic microbeads to compare their xenograft biological characteristics. The results showed that the CD133Hi subgroup of SW620 is more close to the tumor initiating cells in terms of biological characteristics than CD133Mid and CD133low subgroups, but the CD133low subgroup still maintains the ability of tumorigenicity. It supported that tumor initiating cells are more correlated to the abundance of CD133.

著者

• HUANG Xuefeng

  Department of Natural Medicinal Chemistry, China Pharmaceutical University

• Liao Yongqiang

  Biomedical Research Center, Sir Run Run Shaw Hospital, Zhejiang University and Key Laboratory of Bio

• Hu Xiaotong

  Biomedical Research Center, Sir Run Run Shaw Hospital, Zhejiang University and Key Laboratory of Bio

• He Chao

  Biomedical Research Center, Sir Run Run Shaw Hospital, Zhejiang University and Key Laboratory of Bio

• Huang Xuefeng

  Department Of Natural Medicinal Chemistry China Pharmaceutical University

• He Chao

  Biomedical Research Center, Sir Run Run Shaw Hospital, Zhejiang University and Key Laboratory of Biotherapy of Zhejiang Province

• Liao Yongqiang

  Biomedical Research Center, Sir Run Run Shaw Hospital, Zhejiang University and Key Laboratory of Biotherapy of Zhejiang Province

• Hu Xiaotong

  Biomedical Research Center, Sir Run Run Shaw Hospital, Zhejiang University and Key Laboratory of Biotherapy of Zhejiang Province

• Huang Xuefeng

  Department of Colorectal Surgery of Zhejiang University Medical College affiliated Sir Run Run Shaw Hospital

関連論文

• Quantitative Analyses of CD133 Expression Facilitate Researches on Tumor Stem Cells
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