ラット脳細胞の初代培養系を用いてグルコースの取り込み活性でみたアートセレブ®脳脊髄手術用洗浄灌流液の薬理学的評価

概要

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ARTCEREB® irrigation and perfusion solution (Artcereb) is typically used as an artificial fluid for applications inside the skull and spinal cavity. This in vitro study was conducted to assess the effects of Artcereb in cultures of rat fetal brain cells. Cell function following exposure to Artcereb was evaluated by measuring 3H-deoxy-D-glucose incorporation activity. Cell function was significantly reduced in primary cultures of rat fetal brain cells at 0 h and 24 h after 1-h or 3-h exposure to normal saline as compared with Artcereb. Cell function was also significantly reduced at 24 h after 3-h exposure to lactated Ringers solution as compared with Artcereb. Furthermore, cell function was significantly reduced at 24 h after 3-h exposure to a modified Artcereb formulation lacking either HCO3- or Mg2+ as compared with Artcereb, while cell function was unaffected at 24 h after exposure to lactated Ringers solution with HCO3- or normal saline with HCO3- as compared with Artcereb. These findings suggest the importance of the presence of HCO3- and Mg2+ in the formulation of Artcereb.