The Mode of Biofilm Formation on Smooth Surfaces by Campylobacter jejuni
スポンサーリンク
概要
- 論文の詳細を見る
Many microorganisms produce extracellular polymers referred to collectively as "slime" or glycocalyx, and form biofilms on solid surfaces in natural ecosystems. Campylobacter jejuni, one of the most important foodborne pathogens, also has the ability to form biofilm on stainless steel, glass, or polyvinyl chloride in vitro. However, the issue of biofilm formation by Campylobacter species has not been extensively examined. The present study was performed to examine the mode of adhesion of C. jejuni to a smooth surface. When bacterial suspensions in Brucella broth were incubated in microplate wells with a glass coverslip, microcolonies 0.5~2 mm in diameter were formed on the coverslip within 2 hr from the start of incubation. These microcolonies gradually grew and formed a biofilm of net-like connections within 6 hr. Transmission electron microscopy indicated that massive amounts of extracellular material masked the cell surface, and this material bound ruthenium red, suggesting the presence of a polysaccharide moiety. Scanning electron microscopy indicated that the flagella acted as bridges, forming net-like connections between the organisms. To determine the genes associated with biofilm formation, aflagellate (flaA-) and flagellate but non-motile (motA-) mutants were constructed from strain 81-176 by natural transformation-mediated allelic exchange. The flaA- and motA- mutants did not form the biofilm exhibited by the wild-type strain. These findings suggest that flagella-mediated motility as well as flagella is required for biofilm formation in vitro.
- 社団法人 日本獣医学会の論文
著者
-
Moe Kyaw
Laboratory of Veterinary Public Health, Department of Veterinary Science, Faculty of Agriculture, Un
-
Misawa Naoaki
Laboratory Of Animal Microbiology Faculty Of Agriculture Tokyo University Of Agriculture And Technol
-
MIMURA Junichiro
Laboratory of Veterinary Public Health, Department of Veterinary Science, Faculty of Agriculture, Un
-
OHNISHI Takahiro
Laboratory of Veterinary Public Health, Department of Veterinary Science, Faculty of Agriculture, Un
-
WAKE Tomoya
Laboratory of Veterinary Public Health, Department of Veterinary Science, Faculty of Agriculture, Un
-
YAMAZAKI Wataru
Division of Bacteriology, Osaka Prefectural Institute of Public Health
-
NAKAI Masaaki
Laboratory of Veterinary Anatomy, Department of Veterinary Science, Faculty of Agriculture, Universi
-
Moe Kyaw
Laboratory Of Veterinary Public Health Department Of Veterinary Science Faculty Of Agriculture Unive
-
Yamazaki Wataru
Division Of Bacteriology Osaka Prefectural Institute Of Public Health
-
OHNISHI Takahiro
Laboratory of Veterinary Public Health, Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki
-
MIMURA Junichiro
Laboratory of Veterinary Public Health, Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki
-
NAKAI Masaaki
Laboratory of Veterinary Anatomy, Department of Veterinary Science, Faculty of Agriculture, University of Miyazaki
関連論文
- Antimicrobial Susceptibility of Treponema phagedenis-Like Spirochetes Isolated from Dairy Cattle with Papillomatous Digital Dermatitis Lesions in Japan
- Systemic IgA Antibody Responses against Bovine Serum Albumin in the Chicken
- The Mode of Biofilm Formation on Smooth Surfaces by Campylobacter jejuni
- Detection of Treponemes in Canker Lesions of Horses by 16S rRNA Clonal Sequencing Analysis
- Detection of antibodies against Fusobacterium necrophorum and Porphyromonas levii-like species in dairy cattle with papillomatous digital dermatitis