Toward high performance western blotting using vacuum-driven system and laser scanner; comparison between two signal detection methods based on chemiluminescent and immunofluorescent imaging
スポンサーリンク
概要
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A vacuum-driven antibody-reaction system enables high-throughput western blotting, where the antibodies pass through protein-transferred membrane and antibody-antigen reaction is completed within 30 min. With the vacuum-based device, two protein detection methods compatible with by laser scanning were compared; one with enhanced chemiluminescence reaction and the other using fluorescence-labeled antibody. Considering sensitivity, quantitative capability, reproducibility and labor intensiveness, we concluded that fluorescence-based detection is more suitable in combination with the vacuum-driven antibody-reaction system and a laser scanner.
著者
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Harada Chisato
Proteome Bioinformatics Project, National Cancer Center Research Institute
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Tajima Hideji
Research Department, Research & Development Division, Precision System Science
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Hirohashi Setsuo
Proteome Bioinformatics Project, National Cancer Center Research Institute
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Kondo Tadashi
Proteome Bioinformatics Project, National Cancer Center Research Institute
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