Genetic Analysis of the S1 Gene of 4/91 Type Infectious Bronchitis Virus Isolated in Japan

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概要

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• S1 gene sequences for infectious bronchitis virus (IBV) strains of the 4/91 genotype (commonly called 793B) isolated from field outbreaks in Japan were analyzed to ascertain the relationship to 4/91 vaccine strain. Three field isolates (JP/Wakayama/2003, JP/Iwate/2005 and JP/Saitama/2006) from flocks not immunized with a 4/91 type live IBV vaccine and one isolate (JP/Wakayama-2/2004) from a flock immunized with a 4/91 type live vaccine were examined. The amino acid identities among JP/Wakayama/2003, JP/Iwate/2005 and JP/Saitama/2006 were about 98%, whereas the identities to the 4/91 vaccine strain and JP/Wakayama-2/2004 were about 90%. Three of the field isolates, JP/Wakayama/2003, JP/Iwate/2005 and JP/Saitama/2006, were classified into a cluster closely related to French and Spanish isolates, but different from the cluster including the vaccine and JP/Wakayama-2/2004. These results indicate that JP/Wakayama/2003, JP/Iwate/2005 and JP/Saitama/2006 were derived from foreign field isolates, but not from the vaccine strain. On the other hand, the S1 gene of JP/Wakayama-2/2004 revealed high sequence similarity with that of the 4/91 vaccine strain and appeared to be a vaccine-like virus derived from a vaccine. The field isolates of 4/91 genotype IBV could be distinguished from other genotypes by using the BalI and Pst I enzymes in addition to the polymerase chain reaction (PCR) -restriction fragment length polymorphism (RFLP) methods of Mase et al. [16] using Hae II and EcoR I enzymes. Furthermore, the 4/91 vaccine strain and vaccine-like isolate (JP/Wakayama-2/2004) could be differentiated from the other field isolates by Bgl II digestion. This method, therefore, would assist in identification of field isolates of the 4/91 genotype as outbreaks of IBV in vaccinated flocks.

著者

• FUKUDA Masaharu

  Saitama Prefectual Chichibu Highland Farm

• NAKAMURA Shigeyuki

  National Veterinary Assay Laboratory

• Shimazaki Yohko

  National Veterinary Assay Laboratory Ministry Of Agriculture Forestry And Fisheries

• HARADA Makiko

  National Veterinary Assay Laboratory

• SUZUKI Shoko

  National Veterinary Assay Laboratory

• WATANABE Yuuko

  National Veterinary Assay Laboratory

• SEKI Yoshihisa

  Iwate Prefecture Central Livestock Hygiene Service Center

• KURODA Yorifumi

  Wakayama Kihoku Livestock Hygiene Service Center

• HONDA Eiichi

  Tokyo University of Agriculture and Technology

• Honda Eiichi

  Tokyo Univ. Agriculture And Technol. Tokyo

• SHIMAZAKI Yohko

  National Veterinary Assay Laboratory

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