Construction and Characterization of an Actinobacillus pleuropneumoniae Serotype 2 Mutant Lacking the Apx Toxin Secretion Protein Genes apxIIIB and apxIIID

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概要

• 論文の詳細を見る

• Apx toxins have been identified as important virulence factors of <i>Actinobacillus pleuropneumoniae</i>, the etiologic agent of porcine pleuropneumonia. In some <i>A. pleuropneumoniae</i> serotypes, Apx toxins are secreted by the cell membrane proteins encoded by <i>apxIIIB</i> and <i>apxIIID</i> genes. In an effort to develop a live vaccine strain against <i>A. pleuropneumoniae</i>, we inactivated the <i>apxIIIB</i> and <i>apxIIID</i> genes in <i>A. pleuropneumoniae</i> 1536, a serotype 2 strain, resulting in the Δ<i>apxIIIB/DapxIIID</i> mutant strain (1536ΔBΔD). Immunization of pigs with live 1536ΔBΔD <i>A. pleuropneumoniae</i> conferred protection against homologous challenge with wild-type <i>A. pleuropneumoniae</i> 1536. Thus, impaired Apx toxin secretion may decrease the virulence of <i>A. pleuropneumoniae</i> and may be an effective strategy for the development of a live-attenuated <i>A. pleuropneumoniae</i> vaccine.<br>

• 日本獣医学会の論文

著者

• Kim Soohee

  College of Veterinary Medicine, Seoul National University

• Ryu Doug-Young

  College of Veterinary Medicine, Seoul National University

• HA Yooncheol

  College of Veterinary Medicine, Seoul National University

• Park Changbo

  College of Veterinary Medicine, Seoul National University

• CHAE Chanhee

  College of Veterinary Medicine, Seoul National University

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• Involvement of c-Met- and Phosphatidylinositol 3-Kinase Dependent Pathways in Arsenite-Induced Downregulation of Catalase in Hepatoma Cells
• Construction and Characterization of an Actinobacillus pleuropneumoniae Serotype 2 Mutant Lacking the Apx Toxin Secretion Protein Genes apxIIIB and apxIIID

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