Therapeutic Use of Cloning: Osmangazi Turk Identical Embryonic Stem Cells and Embryonic Stem Cell Transfer to Diabetic Mice

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概要

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• There are important problems in the therapeutic use of cloning which increase with the more advanced differentiation of somatic cells. Problems include DNA reprogramming, tissue rejection, and chromatin remodeling. This study presents the first use in somatic cell nuclear transfer (SCNT) of tooth pulp cells containing progenitor cells that resemble the nonaging embryonic connective tissue in adults. Using tooth pulp cells in SCNT increased the first cleavage rates compared with adult somatic cells, leading to a higher rate of DNA reprogramming and increased production of an identical embryonic stem cells (ESCs) line. ESCs derived from tooth pulp cells using this method were observed to transfer into pancreatic β cells using transmission electron microscopy. These cells were named Osmangazi Turk Identical ESCs since this was the first use in SCNT of tooth pulp cells to demonstrate a decrease in glucose levels following administration of these cells. Furthermore, nonidentical ESC perform tissue repair by decreasing glucose levels; however, the renewed tissue was observed to undergo important alterations which affect its future. The prognosis of the therapy of mice with diabetes mellitus using Osmangazi Turk Identical ESC was good, with an 80% therapeutic efficiency. These outcomes are promising for regenerative medicine in the therapy of diabetes mellitus, and clarification of the importance of selecting appropriate adult somatic cells in deriving identical ESC lines is also a significant step.

• 公益社団法人　日本薬学会の論文

著者

• Muslumanoglu Hamza

  Department Of Medical Genetics Osmangazi University Medical Faculty

• Ozden Hilmi

  Department Of Anatomy Osmangazi University Faculty Of Medicine The Central Campus

• Gurer Firdevs

  Department of Histology and Embryology, Faculty of Medicine, University of Eskisehir Osmangazi

• Baycu Cengiz

  Department of Histology and Embryology, Faculty of Medicine, University of Eskisehir Osmangazi

• Cilingir Oguz

  Department of Genetics, Faculty of Medicine, University of Eskisehir Osmangazi

• Hassa Hikmet

  Department of Obstetrics and Gynecology, Faculty of Medicine, University of Eskisehir Osmangazi

• Muslumanoglu Hamza

  Department of Genetics, Faculty of Medicine, University of Eskisehir Osmangazi

関連論文

• Detection of Chromosomal Imbalances in Spinal Meningiomas by Comparative Genomic Hybridization
• Estimation of the Tumor Volume and Volume Ratio on Computed Tomography in Patients with Renal Cell Carcinoma : A Stereological Study
• Therapeutic Use of Cloning: Osmangazi Turk Identical Embryonic Stem Cells and Embryonic Stem Cell Transfer to Diabetic Mice
• Relationship between tumorsize of malignant pleural mesothelioma and its response to chemotherapy

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