Bacillus subtilis Response Regulator DegU Is a Direct Activator of pgsB Transcription Involved in γ-Poly-glutamic Acid Synthesis

スポンサーリンク

概要

• 論文の詳細を見る

• pgsB encodes γ-poly glutamic acid (γ-PGA) synthetase and constitutes an operon with pgsC, pgsAA, and pgsE. Genetic analysis revealed that degQ and swrA, the known regulators of pgsB, are not required for pgsB expression when high cellular concentrations of phosphorylated form of the response regulator DegU (DegU-P) are present. However, swrA appeared still to be required for γ-PGA synthesis under the conditions we tested. Since genetic analysis suggested that DegU-P activates pgsB directly, we performed gel retardation and footprint analyses using purified His-tagged DegU and the pgsB promoter. The in vitro experiments revealed that His-tagged DegU bound to the immediate upstream region of the −35 region of the pgsB promoter. A six-base deletion within the sequence (the −44 to −39 region) abolished DegU-binding to the pgsB promoter and pgsB transcription, confirming the importance of the sequence for DegU-dependent regulation of pgsB. Hence we conclude that DegU is a direct activator of the pgsB operon.

• 社団法人 日本農芸化学会の論文

著者

• OHSAWA Taku

  Institute of Oceanic Research and Development, Tokai University

• TSUKAHARA Kensuke

  Institute of Oceanic Research and Development, Tokai University

• OGURA Mitsuo

  Institute of Oceanic Research and Development, Tokai University

関連論文

• Bacillus subtilis Response Regulator DegU Is a Direct Activator of pgsB Transcription Involved in γ-Poly-glutamic Acid Synthesis
• Superoxide Stress Decreases Expression of srfA through Inhibition of Transcription of the comQXP Quorum-Sensing Locus in Bacillus subtilis
• Identification of the Sequences Recognized by the Bacillus subtilis Response Regulator YrkP
• Bacillus subtilis Response Regulator DegU Is a Direct Activator of pgsB Transcription Involved in γ-Poly-glutamic Acid Synthesis

スポンサーリンク