A Long-Wavelength, Fluorogenic Probe for Epoxide Hydrolase: 7-(2-(Oxiran-2-yl)ethoxy) Resorufin
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概要
- 論文の詳細を見る
Epoxide hydrolase plays an important role in the detoxification of genotoxic compounds and in the control of physiological signaling molecules. Altered levels of epoxide hydrolase activity are associated with many diseases, such as emphysema, lung cancer, ovarian cancer, and laryngeal carcinoma. We designed and synthesized a resorufin-based fluorogenic probe, 7-(2-(oxiran-2-yl)ethoxy) resorufin, which was hydrolyzed by microsomal epoxide hydrolase to form the corresponding diol, which upon further treatment with sodium periodate released the strongly fluorescent resorufin. The probe exhibits good biological compatibility and photophysical properties, such as long wavelength excitation (571 nm) and emission (585 nm) and a wide working pH range (from 6.0 to 10.0), and thus facilitates the determination of the activity of microsomal epoxide hydrolase.
- 公益社団法人 日本薬学会の論文
著者
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Shen Wei
Department Of Cardiology Rui Jin Hospital Shanghai Jiao Tong University School Of Medicine
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Zhu Qing
Institute of Bioengineering, Zhejiang University of Technology (Chaohui Campus)
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Zhang Jiaming
Department of General Surgery, Jinhua Central Hospital
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Mao Genjun
Department of General Surgery, Jinhua Central Hospital
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Jiang Keyi
Institute of Bioengineering, Zhejiang University of Technology (Chaohui Campus)
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ZHU Qing
Institute of Animal Genetics and Breeding, Sichuan Agricultural University
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