Antinociceptive Effect of Crude Extract, Fractions and Three Alkaloids Obtained from Fruits of Piper tuberculatum

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This study investigated the possible antinociceptive effect of the crude extract, fractions and pure compounds (three alkaloids) obtained from fruits of Piper tuberculatum JACQ. (Piperaceae) in acetic acid-induced visceral pain in mice. Oral administration of crude extract and fractions (CH2Cl2, EtOAc, methanol and hexane) (3—300 mg kg−1) caused a dose-related and significant inhibition of the acetic acid-induced visceral nociceptive response. The crude extract, dichloromethane (CH2Cl2) and ethyl acetate (EtOAc) fractions were more potent than methanol and hexane fractions. The isolated alkaloids dihydro-piplartine, piplartine and 3,4,5-trimethoxydihydrocinnamic acid (0.0001—30 mg kg−1) exhibited significant and dose-related antinociceptive effects against acetic acid-induced visceral pain. The results show, for the first time, that crude extract, fractions and pure compounds obtained from P. tuberculatum produce marked antinociception against the acetic acid-induced visceral nociceptive response, supporting the ethnomedical use of P. tuberculatum.