Evaluation of the Cytotoxicity Effect of Dimethyl Sulfoxide (DMSO) on Caco2/TC7 Colon Tumor Cell Cultures

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概要

• 論文の詳細を見る

• Dimethyl sulfoxide (DMSO) is usually used to solubilize poorly soluble drugs in permeation assays such as that using Caco2 enterocyte-like cells. The objective of this study was to evaluate the toxicity of DMSO on Caco2/TC7 cells and determinate the maximal concentration usable in permeation experiments. Caco2/TC7 cells were cultured for 21 d on 96-well plates for evaluation of toxicity. The determination of lactate dehydrogenase (LDH) release in cell supernatant and the measurement of Neutral Red (NR) uptake are used for cytotoxicity assays. DMSO solutions (0—100%) in Hanks balanced salt solution containing HEPES (25 mM), pH 7.4, were incubated with Caco-2/TC7 cells on 96 well plates. Caco2/TC7 cells were cultured on Transwell-Clear® inserts to evaluate the influence of DMSO on the apparent permeability of the paracellular marker mannitol. DMSO 10% did not induce any significant increase in LDH release whereas a significant increase in LDH activity (ANOVA, p<0.05) occurred at a DMSO concentration of 20 to 50%. NR incorporation in viable cells was statistically reduced by 27 to 36% at DMSO concentration of 20% up to 100% (ANOVA, p>0.05). No statistical difference (p<0.05) in apparent mannitol permeability was observed between the control and 10% DMSO groups. In conclusion, at concentrations of up to 10%, DMSO did not produce any significant alteration in apical membrane permeability or on cell-to-cell tight junctional complexes.

• 公益社団法人　日本薬学会の論文
• 2002-12-01

著者

• Arnaud Philippe

  Service Commun De Microscopie Electronique Faculte Des Sciences Pharmaceutiques Et Biologiques Unive

• Provot Gerard

  Laboratoire Glaxosmithkline New Chemical Entity Unit

• VIOLANTE Georges

  Laboratoire de Pharmacie Galenique, UPRES EA 2498, Faculte des Sciences Pharmaceutiques et Biologiqu

• ZERROUK Naima

  Laboratoire de Pharmacie Galenique, UPRES EA 2498, Faculte des Sciences Pharmaceutiques et Biologiqu

• RICHARD Isabelle

  Laboratoire GlaxoSmithKline, New Chemical Entity Unit

• CHAUMEIL Jean

  Laboratoire de Pharmacie Galenique, Faculte des Sciences Pharmaceutiques et Biologiquea, UPRESS EA 2

• ARNAUD Philippe

  Laboratoire de Pharmacie Galenique, Faculte de Medecine et de Pharmacie, ADEN EA 3234, Universite de

• Arnaud Philippe

  Laboratoire De Pharmacie Galenique Aden Ea 3234 Faculte De Medicine Pharmacie

• Zerrouk Naima

  Laboratoire De Pharmacie Galenique Upres Ea 2498 Faculte Des Sciences Pharmaceutiques Et Biologiques

• Violante Georges

  Laboratoire De Pharmacie Galenique Upres Ea 2498 Faculte Des Sciences Pharmaceutiques Et Biologiques

• Richard Isabelle

  Laboratoire Glaxosmithkline New Chemical Entity Unit

• Chaumeil Jean-claude

  Unite Rech. Et Developpement Galenique Agence Generale Des Equipements Et Produits De Sante

• Richard Isabelle

  Laboratoire D'epidemiologie Ergonomie Et Sante Au Travail University Of Angers

• Da Violante

  Laboratoire de Pharmacie Galenique, UPRES EA 2498, Faculte des Sciences Pharmaceutiques et Biologiques, Universite Rene Descartes

• Arnaud Philippe

  Laboratoire de Pharmacie Galénique, Faculté de Médecine et de Pharmacie, ADEN EA 3234, Université de Rouen

• Chaumeil Jean

  Laboratoire de Pharmacie Galénique, Faculté des Sciences Pharmaceutiques et Biologiques, UPRESS EA 2498, Université René Descartes

• Zerrouk Naima

  Laboratoire de Pharmacie Galénique, Faculté des Sciences Pharmaceutiques et Biologiques, UPRESS EA 2498, Université René Descartes

関連論文

• Short Term Caco-2/TC7 Cell Culture : Comparison between of Conventional 21-d and a Commercially Available 3-d System(Biopharmacy)
• Evaluation of the Cytotoxicity Effect of Dimethyl Sulfoxide (DMSO) on Caco2/TC7 Colon Tumor Cell Cultures
• Effect of iontophoresis and penetration enhancers on transdermal absorption of metopimazine
• Multidisciplinary Intensive Functional Restoration Versus Outpatient Active Physiotherapy in Chronic Low Back Pain

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