Physico-chemical characterization and biocompatibility evaluation of hydroxyapatites

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概要

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• The aim of this study was to evaluate the physico-chemical and biocompatibility characteristics of two different hydroxyapatites. Physical and chemical properties were analyzed using granulometric analysis, scanning electron microscopy (SEM), X-ray energy-dispersion (EDX), X-ray fuorescence (XRF) and X-ray diffraction (XRD). Biomaterials were implanted into the subcutaneous tissue on the dorsum of 36 Wistar rats, divided into the following groups: Group 1 – Gen-Ox™ (natural); Group 2 – HA-U (synthetic) and Group 3 – Control (Sham). After 15 and 30 days, 6 animals/period were sacrificed and the subcutaneous tissue was taken for histological and histometric analysis, giving consideration to inflammatory reaction and granule area. The granulometric test results showed a mean granule diameter of 161.6 μm (min = 19.0 μm; max = 498.0 μm) and 48.7μm (min = 7.0 μm; max = 256.0 μm) for groups 1 and 2 respectively. Analysis with SEM demonstrated irregular and sharp-edge particles in group 1 (3332.8 ± 274.3 μm<Sup>2</Sup>) and irregular and rounded particles in group 2 (1320.8 ± 83.0 μm<Sup>2</Sup>) (<I>P</I> < 0.0001; Student's <I>t</I> test). EDX and XRF revealed calcium, carbon, oxygen, sodium and phosphorus in both groups. XRD indicated that both biomaterials were pure and crystalline. There was a statistically significant difference in granule area between the two groups after 15 days (<I>P</I> = 0.022; Student's <I>t</I>-test). After 15 days, an increased inflammatory response was seen in group 2 (<I>P</I> < 0.0001; ANOVA and Tukey's post hoc test) whereas it was more pronounced in group 1 after 30 days (<I>P</I> < 0.0001; ANOVA and Tukey's post hoc test). It was concluded that these biomaterials have similar physical, chemical and biocompatibility characteristics. (J. Oral Sci. 48, 219-226, 2006)

• 日本大学歯学部の論文

著者

• Silva Jose

  Departamento De Biologia Celular E Genetica Universidade Estadual De Maringa

• Cruz Ariadne

  Department of Dentistry, State University of Ponta Grossa

• Pilatti Gibson

  Department of Dentistry, Ponta Grossa State University

• Santos Fábio

  Department of Dentistry, Ponta Grossa State University

• Pochapski Márcia

  Department of Dentistry, State University of Ponta Grossa

• Daher Josélia

  Department of Pharmacy, State University of Ponta Grossa

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