Rapid Production of Candida albicans Chlamydospores in Liquid Media under Various Incubation Conditions

概要

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The production of chlamydospores is a diagnostic tool used to identify Candida albicans; these structures also represent a model for morphogenetic research. The time required to produce them with standard methods is 48-72 hours in rice meal agar and tensoactive agents. This time can be shorted using liquid media such as cornmeal broth (CMB) and dairy supplements.Five media were tested: CMB plus 1% Tween-80, CMB plus 5% milk, CMB plus 5% milk serum, milk serum, and milk serum plus 1% Tween-80, under different incubation conditions: at 28°C and 37°C in a metabolic bath stirring at 150rpm, and at 28°C in a culture stove. The reading time points were established at 8 and 16 hours. The best results were obtained at 16 hours with CMB plus 5% milk under incubation at 28°C and stirring at 150 rpm. The next most efficient methods were CMB plus 5% milk serum and CMB plus 1% Tween-80, under the same incubation conditions. The other media were ineffective in producing chlamydospores. The absence of stirring at 28°C prevented the formation of chlamydospores within the set time points, and incubation at 37°C decreased their production.This paper reports that the time to form C. albicans chlamydospores can be reduced.
日本医真菌学会の論文
2006-07-31