Immunoelectron Microscopic Examination of Cord Blood CD5^+ B cells

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In this study, we have categorized cord blood CD5+ B cells, which were examined using the horseradish peroxidase-colloidal gold double labeling immunoelectron microscopy, into three subtypes based on their morphology and immunohistochemical characteristics. Type 1a cells and type 1b cells (9% and 17% of the CD5+ B cells, respectively) had few cytoplasmic organelles, a high nuclear/cytoplasmic (N/C) ratio (0.66 ± 0.03 and 0.58 ± 0.04, respectively), and a low nuclear contour index (NCI) value (1.56 ± 0.30 and 1.50 ± 0.27, respectively), whereas type 2 cells (74% of the CD5+ B cells) had a low N/C ratio (0.44 ± 0.11) and a high NCI value (2.05 ± 0.68). Type 2 cells, which had many cytoplasmic organelles, frequently had several uropod-like processes that bound to the gold particles. The N/C ratios clearly showed that there were significant differences among the three types of CD5+ B cells (p < 0.01), and between CD5+ T cells and the three types of CD5+ B cells (p < 0.05). For the NCI values, only type 1b and type 2 cells showed a significant difference (p < 0.05). These findings suggest that type 1a cells are transformed into type 1b cells, and then into type 2 cells. [J Clin Exp Hematopathol 46(2) : 83-88, 2006]
日本リンパ網内系学会の論文
2006-11-01