Rice OASA1D, a mutant anthranilate synthase α subunit gene, is an effective selectable marker for transformation of Arabidopsis thaliana

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We have previously developed an antibiotic-free system for the selection of plant transformants that is based on a gene (OASA1D) for a mutant α subunit of rice anthranilate synthase. The product of this gene shows a reduced sensitivity to negative feedback regulation by tryptophan. Whereas 5-methyltryptophan (5MT) is lethal for normal plant cells because it causes tryptophan deficiency, expression of OASA1D confers resistance to this tryptophan analog. We used this OASA1D-5MT system for the transformation of Arabidopsis thaliana. An expression vector containing OASA1D under the control of the 35S promoter of cauliflower mosaic virus was introduced into Arabidopsis by Agrobacterium-mediated transformation. Transgenic plants that harbored OASA1D exhibited resistance to 5MT but did not manifest any other differences in growth, morphology, or fertility. The OASA1D-5MT selection system performed as well as the HPT-hygromycin system for the transformation of Arabidopsis. Given the limited number of conventional marker genes currently available, OASA1D should prove to be a useful tool in Arabidopsis transformation, especially for the generation of plants carrying multiple transgenes.
日本植物細胞分子生物学会の論文